Structural Similarities between Glutamate Receptor Channels and K+ Channels Examined by Scanning Mutagenesis
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چکیده
منابع مشابه
Structural Similarities between Glutamate Receptor Channels and K 1 Channels Examined by Scanning Mutagenesis ✪
The pores of glutamate receptors and K 1 channels share sequence homology, suggesting a conserved secondary structure. Scanning mutagenesis with substitution of alanine and tryptophan in GluR6 channels was performed based on the structure of KcsA. Our assay used disruption of voltage-dependent polyamine block to test for changes in the packing of pore-forming regions. Alanine scanning from D567...
متن کاملStructural Similarities between Glutamate Receptor Channels and K+ Channels Examined by Scanning Mutagenesis
The pores of glutamate receptors and K(+) channels share sequence homology, suggesting a conserved secondary structure. Scanning mutagenesis with substitution of alanine and tryptophan in GluR6 channels was performed based on the structure of KcsA. Our assay used disruption of voltage-dependent polyamine block to test for changes in the packing of pore-forming regions. Alanine scanning from D56...
متن کاملAMPA Glutamate Receptor Channels
The channel of the glutamate N -methyld -aspartate receptor (NMDAR) transports Ca 2 1 approximately four times more efficiently than that of Ca 2 1 -permeable a -amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors (AMPAR). To investigate the basis of this difference in these glutamate receptors (GluRs), we measured the ratio of Cs 1 efflux and Ca 2 1 influx in recombinant NMDAR and Ca 2 1 ...
متن کاملDifferent gating mechanisms in glutamate receptor and K+ channels.
The basic structural features of channel gating in glutamate receptors (GluRs) remain unknown. Here we used covalent modification of substituted cysteines and fast agonist application to study the contribution of the M3 segment in AMPA receptor GluR-A subunits to channel structure and gating. The pattern of accessibility of substituted cysteines to extracellularly applied methanethiosulfonate r...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 2001
ISSN: 0022-1295,1540-7748
DOI: 10.1085/jgp.117.4.345